human brain capillary endothelial cells hbmec  (Innoprot Inc)

Journal:

Article Title: Anti-Tumoral Activity of a Short Decapeptide Fragment of the Alzheimer's A? Peptide

doi: 10.1007/s10989-010-9198-8

Figure Lengend Snippet: Effect of 4-hydroxy tamoxifen on angiogenesis in vitro. a Representative pictures depicting the formation of capillary networks in response to a dose range of 4-hydroxy tamoxifen following the plating of human brain microvascular endothelial cells onto a layer of Matrigel. b The graph represents the quantification of capillary network length by image analysis and the amount of LDH activity detected in the culture medium surrounding the capillary like structures following 24 h of treatment with a dose range of 4-hydroxy tamoxifen. ANOVA revealed a significant main effect of 4-hydroxy tamoxifen on LDH released (P < 0.03) but no significant main effect on capillary formation (P = 0.743) and post hoc analysis showed significant differences for LDH released between control conditions and 10 µM of 4-hydroxy tamoxifen (P < 0.009)

Article Snippet: Capillary Morphogenesis Assay Human Brain Microvascular Endothelial Cells (HBMEC) (Sciencell, CA) were cultured in Endothelial Cell Growth Medium (Cell Applications, CA) containing 5% fetal bovine serum, 1% penicillin/streptomycin and 1% Endothelial Cell Growth Supplement (Sigma–Aldrich, MO).

Techniques: In Vitro, Activity Assay, Control